Hi,
Please, give me more details what you did to get ORFs then I can help you. Maybe you didn’t set up the size of minimal ORF in the corresponding textfield ? Without this value the app cannot search for ORFs. Also, the search will be perform if user selects the region of search in the sequence.
Best regards
I am considering to purchase BiolabDonkey. I do a lot of cloning experiments. Can this software be used to perform insilico cloning, restriction digestion, and ligation reactions? Let me know.
Thank You
Hello Krishna Kurthkoti,
In BioLabDonkey you can perform a restriction analysis – find positions of restriction sites (present, not present, number of cuts) in a real DNA sequence. If you mean as insilco cloning a graphical representation of cloning scheme of your insert into a vector like in SnapGene program, BioLabDonkey is not providing such functionality. In BioLabDonkey you should copy/paste your insert sequence into a vector sequence in the corresponding restriction sites. BioLabDonkey provides a virtual DNA gel of digested DNA fragment for the restrictases you selected. In BioLabDonkey you can also calculate amounts of insertion and vector needed for a ligation reaction for different insertion/vector ratios.
If you have further questions, let me know.
Best regards,
Valeriy
Hi Krishna Kurthkoti,
Before switching to program developing, I have spent more than 20 year in molecular biology wet lab doing a lot of cloning and, IMO, I see usage of such a graphical cloning not good for the professional education. Dealing with a DNA sequence directly gives you solid skills while a graphical cloning is too superficial. So, the implementation of such a functionality has lowest priority, not for near future.
Best regards,
Valeriy
I just purchased the BioLabDonkey and it crashed instantly when I tried to open a file (.dna file, created by SnapGene). I tried a second file, crashed, tried a third file, crashed again. Every time I sent the crash report to Apple. What is going on? Brand new Macbook pro, OS10.15.6, 16g RAM.
Hi,
I don’t have any crash report from Apple! Please, send me to my e-mail box a crash report and a file (if it is possible) you have tried to open, that I can fix the issue as soon as possible!
When I had made the function to open SnapGene .dna files, I have tested original SnapGene .dna files dowloaded from official SnapGene website – Basic Cloning Vectros – https://www.snapgene.com/resources/plasmid-files/?set=basic_cloning_vectors. I can open all of them without any problem. What kind of SnapGene .dna files you are trying to open ?
Hi Valeriy, I reported that BioLabDonkey crashes every time when I tried to open .dna file. I have sent you an email including the crash report and some .dna files that always lead to crash. Thank you!
Dear Users, please pay attention if you are trying to open a .dna file from SnapGene. You can have a problem if this file doesn’t have a date of creation in the note section. This issue will be fixed soon in a next update.
I want to contig with two ab1 files. In the sequence columns, when I select unclear region (such as NNN), if the chromatogram data are automatically pop-uped and confirm the peal data, it is easy to confirm and modified and change the sequence. I significantly asked you to update like this.
Hi,
Thank you for your feedback, it allows to improve the app ! I have just updated the function you have asked for. I will update the app in AppStore as soon as possible!
Best regards,
Valeriy
I have been serial cloner user until Apple decided to stop 32-bit programs. Do you offer the capabilities to do virtual PCR, Gateway/recombination cloning? Not having a trial version is such a bummer, can’t just purchase a software that does not have extensive documentation/user base without having tried it. Thank you for your work though!
Hi, there are no options for virtual PCR, Gateway/recombination cloning.
Frankly to say I don’t see a sense in virtual PCR. From my experience with PCR in wet lab (more then 20 years) to have a good result of a real PCR you don’t need virtual PCR, waste of time. It is just matter of choice of good primers and polymerase preset.
I can’t get it to find ORFs in my plasmid sequence.
LikeLike
Hi,
Please, give me more details what you did to get ORFs then I can help you. Maybe you didn’t set up the size of minimal ORF in the corresponding textfield ? Without this value the app cannot search for ORFs. Also, the search will be perform if user selects the region of search in the sequence.
Best regards
LikeLike
Hello,
I am considering to purchase BiolabDonkey. I do a lot of cloning experiments. Can this software be used to perform insilico cloning, restriction digestion, and ligation reactions? Let me know.
Thank You
LikeLike
Hello Krishna Kurthkoti,
In BioLabDonkey you can perform a restriction analysis – find positions of restriction sites (present, not present, number of cuts) in a real DNA sequence. If you mean as insilco cloning a graphical representation of cloning scheme of your insert into a vector like in SnapGene program, BioLabDonkey is not providing such functionality. In BioLabDonkey you should copy/paste your insert sequence into a vector sequence in the corresponding restriction sites. BioLabDonkey provides a virtual DNA gel of digested DNA fragment for the restrictases you selected. In BioLabDonkey you can also calculate amounts of insertion and vector needed for a ligation reaction for different insertion/vector ratios.
If you have further questions, let me know.
Best regards,
Valeriy
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Thanks for the clarification Valeriy. Are you considering adding such functionality in future releases?
LikeLike
Hi Krishna Kurthkoti,
Before switching to program developing, I have spent more than 20 year in molecular biology wet lab doing a lot of cloning and, IMO, I see usage of such a graphical cloning not good for the professional education. Dealing with a DNA sequence directly gives you solid skills while a graphical cloning is too superficial. So, the implementation of such a functionality has lowest priority, not for near future.
Best regards,
Valeriy
LikeLike
I just purchased the BioLabDonkey and it crashed instantly when I tried to open a file (.dna file, created by SnapGene). I tried a second file, crashed, tried a third file, crashed again. Every time I sent the crash report to Apple. What is going on? Brand new Macbook pro, OS10.15.6, 16g RAM.
LikeLike
Hi,
I don’t have any crash report from Apple! Please, send me to my e-mail box a crash report and a file (if it is possible) you have tried to open, that I can fix the issue as soon as possible!
When I had made the function to open SnapGene .dna files, I have tested original SnapGene .dna files dowloaded from official SnapGene website – Basic Cloning Vectros – https://www.snapgene.com/resources/plasmid-files/?set=basic_cloning_vectors. I can open all of them without any problem. What kind of SnapGene .dna files you are trying to open ?
Best regards,
Valeriy
LikeLike
Hi Valeriy, I reported that BioLabDonkey crashes every time when I tried to open .dna file. I have sent you an email including the crash report and some .dna files that always lead to crash. Thank you!
LikeLike
Solved.
LikeLike
Dear Users, please pay attention if you are trying to open a .dna file from SnapGene. You can have a problem if this file doesn’t have a date of creation in the note section. This issue will be fixed soon in a next update.
LikeLike
Solved.
LikeLike
I want to contig with two ab1 files. In the sequence columns, when I select unclear region (such as NNN), if the chromatogram data are automatically pop-uped and confirm the peal data, it is easy to confirm and modified and change the sequence. I significantly asked you to update like this.
LikeLike
Hi,
Thank you for your feedback, it allows to improve the app ! I have just updated the function you have asked for. I will update the app in AppStore as soon as possible!
Best regards,
Valeriy
LikeLike
I have been serial cloner user until Apple decided to stop 32-bit programs. Do you offer the capabilities to do virtual PCR, Gateway/recombination cloning? Not having a trial version is such a bummer, can’t just purchase a software that does not have extensive documentation/user base without having tried it. Thank you for your work though!
LikeLike
Hi, there are no options for virtual PCR, Gateway/recombination cloning.
Frankly to say I don’t see a sense in virtual PCR. From my experience with PCR in wet lab (more then 20 years) to have a good result of a real PCR you don’t need virtual PCR, waste of time. It is just matter of choice of good primers and polymerase preset.
LikeLike