Info/Calculators

  • Dilution calculator
  • Molarity calculator
  • Ligation calculator
  • SDS PAG calculator
  • Nucleic acid OD conversion
  • Ammonium sulfate calculator
  • Bacteria growth calculator
  • Units conversion

Dilution calculator
Use this calculator to calculate the amount of stock solution needed to prepare a solution of the desired molar concentration. 

Molarity calculator
Use this calculator to calculate the required amount of chemical substance (in gram) to add in order to prepare a solution of desired molar concentration.

Ligation calculator
Use this to calculate the required amounts of vector and insert DNA needed to achieve a particular ratio of insert to vector.

SDS PAG calculator
Use this calculator to calculate the amounts of components needed for preparing the separating and stacking gels of a discontinuous gel.

Nucleic acid OD conversion
Use this calculator to calculate the concentration of nucleic acids in a solution (in microgram per millilitre). Path length is the width of cuvette, which is commonly 10 mm (1 cm). 

Ammonium sulfate calculator
Use this calculator to calculate the final volume of the solution and the amount of sulfate (in gram) needed to add to the initial solution. The calculation can be performed for different temperatures. 
The calculator is based on the info from the following publication. Paul T. Wingfield. Protein Precipitation Using Ammonium Sulfate. Curr Protoc Protein Sci. 2001 May. APPENDIX 3: Appendix–3F.doi:10.1002/0471140864.psa03fs13.

Bacteria growth calculator (for the exponential phase) 
Use this calculator to estimate the time required until a desired cell density is reached (expected OD). 

Units conversion
Use this calculator to convert gram to mol, and mol to gram, as well as to calculate the corresponding number of molecules of the nucleic acid or protein.

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